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Cytology

                                                      Enzymes are complex, three dimensional
                                                      globular proteins that are produced in the
                                                      cells  of organisms.  They are biological
                                                      catalysts,  since  they  alter  the  rate  of
                                                      different physiological processes in living
                                                      organisms. They are also found in natural
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                                                      secretions, such as plant juices, milk and
                         (a)        (b)       (c)      (d)  the digestive  juices  where they catalyse
               Figure 1.41: Test tubes with sample    several  metabolic  reactions  or processes
                solutions in different concentration  taking place in the living cells.  A compound
                                                      with which the enzyme combines is called
           (a)  Based on the observations, which test   a substrate. The substrate fixes itself in an
                tube had a food item with maximum
                protein content?                      active site of the enzyme whose shape is
                                                      ideal  for accommodating  it.  The  active
           (b) Classify the proteins based on their  site is a group of amino acids comprising
                structure and function                the region of the enzyme  into which

           1.4.4 Enzymes                              the substrate fits in order to catalyse the
                                                      reaction. By acting as catalysts, enzymes
             Task 1.7                                 lower the activation  energy.  Activation
             Search  from internet  sources the       energy is the minimum amount of energy
             simulations  for  activation  energy  in   that is required to activate  a substrate
             enzymic reaction. Note down  the         molecule  to a condition in which it can
             activation energy of the reactants with   undergo biological transformation (Figure
             an enzyme and without enzyme.            1.42).







                                                            Activation energy (EA)
                                                            without enzymes

                                                            Activation energy (EA)
                                                            with enzymes
                         Free energy (G)  Energy of                  Change in

                               reactants
                                                                     free energy
                                                                     (AG)



                                                Energy of products
                                         Progress of reaction

               Figure 1.42: Activation energy for an enzyme-catalysed reaction and an un-catalysed reaction


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