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Cytology

           Vacuoles                                      cell   (autolysis).  Plant   vacuoles
           Vacuoles are membrane bounded sacs,           can function  in the same way as in
           which are  found within  the  cytoplasm       lysosomes of animal cells as they both
           of a cell. The vacuoles of plant cells are    participate in an automatic cell death
           bounded by single  membranes  called          by autolysis. This process occurs when
           tonoplast.  These  vacuoles  are  formed      the tonoplast ruptures and releases its
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           when vesicles released by the endoplasmic     contents (enzymes) into the cytoplasm
           reticulum  and  Golgi  apparatus  merge       which digests the entire cell.
           together. Young plants consist of a number  (d) They play vital role in primary growth,
           of smaller vacuoles, which during growth      since the pressure exerted by fusion
           and development,  fuse to form a large        of small vacuoles leads to elongation
           central permanent vacuole. Therefore, in      of the cells. Hence, this increases the
           a mature plant cell, cytoplasm is displaced   length of a plant organ.
           and seen as a thin layer near the periphery  (e) They contain anthocyanins, which
           of the cell  surrounding the large central    provide colour to  flowers, fruits,
           vacuole.  The  central  positioning  of  the   and  buds. These  pigments  facilitate
           vacuole pushes the nucleus more or less       pollination and seed dispersal.
           to the periphery or near to the cell surface
           membrane. At this stage, the vacuole is    Ribosomes
           filled with cell sap, which contains water,   Ribosomes are tiny organelles found in
           phenol, mineral salts, alkaloids, pigments,   the matrix of mitochondria, chloroplasts,
           sugars, and proteins.  Animal  cell  lack   and cytoplasm of the cells. They occur in
           vacuoles, but if present they are small    both, prokaryotic and eukaryotic cells. In
           and temporary, associated with storage of   prokaryotic cells they are found freely in
           food and other  materials  to be secreted,   the cytoplasm, whereas in the eukaryotic
           transported  or  removed.  An example      cells they are either attached to the outside
           of vacuoles is  food vacuole or vesicle    of the endoplasmic reticulum (or nuclear
           (phagocytotic or pinocytotic vesicles).    envelope)  or they  occur freely  in the
                                                      cytoplasm. Structurally, each ribosome is
           Functions of vacuoles                      made up of two sub-units, a small sub-unit
           Vacuoles in plant cells play the following   and a large sub-unit (Figure 1.13). Basing
           roles:                                     on their size and sedimentation coefficient,
           (a) They  support  herbaceous  plants  and   there are two types of ribosomes. These
               herbaceous parts of woody plants by    are:
               providing  an  osmotic  system  which  (a)  70s ribosomes:  These are relatively
               creates a pressure potential.             smaller, with sedimentation coefficient
           (b) They act as temporary  stores for         of 70s and  molecular weight of  2.7x10 6
               different  substances,  such as food,     Daltons.  They occur in prokaryotic
               enzymes, and waste materials.             cells, such as in blue-green algae and
                                                         bacteria.They also occur in eukaryotic
           (c) They have hydrolytic enzymes which        cells organelles, such as mitochondria
               destroy dead cells or the entire plant    and chloroplasts.


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