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Gas exchange and respiration

           triose phosphate produced in the lysis of
           Fructose-1, 6-biphosphate.  But the  two   Exercise 6.2
           ATP formed are used to pay back the         1.  Discuss the  role  of enzymes in a
           initial ATP  used  during  the  first  process   glycolytic pathway.
           of glycolysis (phosphorylation of glucose   2.  What  would  happen if  the  first
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           stage).                                        enzyme in glycolysis is irreversibly
                                                          inhibited by a toxic substance?
           The  formed  3-PGA is converted  into
           2-phosphoglycerate or 2-phosphoglyceric     3.  Starting  with   glucose,   write
                                                          the  overall  reaction  for  aerobic
           acid  (2-PGA) by phosphoglyceromutase          respiration.
           (phosphoglycerate    mutase)    enzyme.
           This enzyme rearranges the phosphate        4.  What are the main stages of aerobic
           group from the third carbon of 3-PGA           respiration? List down the important

           to the second carbon,  hence  forming          materials that are involved in each
                                                          stage.
           2-PGA.  The 2-phosphoglycerate  is then
           converted  into  phosphoenol pyruvate       5.  The initial  stages of glycolysis
           (PEP)  under  the  influence  of  enolase      involve the use of ATP.  Explain.
           enzyme, in this reaction water molecule is   6.  Explain the mechanism of gas
           removed from 2-phosphoglycerate. Then          exchange in plants.
           the phosphoenol pyruvate  is converted
           into pyruvate (pyruvic acid) by pyruvate   6.3.3 The fate of pyruvic acid
           kinase enzyme, where by the phosphate      The pyruvate produced during glycolysis

           group (Pi) from PEP is transferred to      has two possible fates, depending on the
           ADP molecule and combine to form           availability of oxygen in the cell. In the
           ATP molecule.  Again two molecules of      presence  of oxygen, the  pyruvate  will
           ATP and two pyruvate are formed as         enter Kreb’s cycle in which they will be
           explained  earlier.  This is because the   completely  oxidised into carbon dioxide
           triose phosphate has to enter twice in the   and water.  Alternatively, in the absence
           glycolysis process that is; when 3-PGAL    of oxygen  (in anaerobic  condition)  the

           is used, the dihydroxyacetone phosphate    pyruvate will undergo fermentation.
           is converted into 3-PGAL and enters the
           cycle. The formation of pyruvate ends the
           glycolysis process (Figure 6.9).









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